Lexogen Features Insight on Deriving High-resolution RNA Maps for the Regulation of Alternative Polyadenylation

Gregor Rot, PhD Postdoctoral Researcher, Institute of Molecular Life Sciences University of Zurich; Jernej Ule, PhD Group Leader, The Francis Crick Institute;

Many RNA binding proteins (RBPs) regulate the selection of alternative polyA sites. To understand their regulatory principles, the research team developed expressRNA, a web platform encompassing computational tools for integration of the QuantSeq 3´ mRNA-Seq, iCLIP and RNA motif analyses. This reveals at nucleotide resolution the ‘RNA maps’, which demonstrate that RBPs bind to specific positions on pre-mRNAs to regulate the polyA sites.

The RNAmotifs2 software also identifies clustered sequence motifs that mediate the regulation of these sites. The team used this approach to show that TDP-43, an RBP involved in several neurodegenerative diseases, binds close to the polyA site to repress, and further downstream to enhance their use. Conclusions were made that TDP 43 directly regulates diverse types of pre-mRNA processing events according to common positional principles.

Through this webinar, sponsored by Lexogen, attendees will learn how to use the expressRNA platform and analyse 3´ mRNA-Seq QuantSeq data. They will also learn why is it helpful to derive an RNA map, and what does it show us about regulatory mechanisms.

This webinar will have two speakers; Dr. Jernej Ule, group leader at the Francis Crick Institute, and Dr. Gregor Rot, a postdoctoral researcher at the Institute of Molecular Life Sciences, at the University of Zurich.

Ule received a doctorate in molecular neuroscience from the Rockefeller University, New York, and started his research group at the MRC Laboratory of Molecular Biology in Cambridge. In 2013, he moved with his group to the Institute of Neurology at…

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